Recombinant Human Desmoglein-2

Desmoglein 2, also known as DSG2, is a human gene. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. It provides cellular adhesion and forces transduction by cell-to-cell anchorage. ¹ It also plays a role in embryonic stem cell proliferation.

The biological activity of Human DSG2 was determined by the ability of the immobilized protein to support the adhesion of BUD8 human skin fibroblast cells. When 3 x 10⁴ cells are added to recombinant human Desmoglein 2 coated plates (10 μg/ml, 100 μl/well), approximately 50%-80% will adhere after 1 hour at 37°C.

Fc Fusion Protein

awitapvalr egedlskknp iakihsdlae erglkityky tgkgiteppf gifvfnkdtg elnvtsildr eetpfflltg yaldargnnv ekplelrikv ldindnepvf tqdvfvgsve elsaahtlvm kinatdadep ntlnskisyr ivslepaypp vfylnkdtge iyttsvtldr eehssytltv eardgngevt dkpvkqaqvq irildvndni pvvenkvleg mveenqvnve vtrikvfdad eigsdnwlan ftfasgnegg yfhietdaqt negivtlike vdyeemknld fsvivankaa fhkscdktht cppcpapell ggpsvflfpp kpkdtlmisr tpevtcvvvd vshedpevkf nwyvdgvevh naktkpreeq ynstyrvvsv ltvlhqdwln gkeykckvsn kalpapiekt iskakgqpre pqvytlppsr deltknqvsl tclvkgfyps diavewesng qpennykttp pvldsdgsff lyskltvdks rwqqgnvfsc svmhealhnh ytqkslslsp gk

Ala49

Lyophilized

The predicted molecular weight of Recombinant Human Desmoglein-2 is Mr 89 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 115 kDa.

89

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Lieber, Andre´ et al. (2011) J Virol. 85(13):6390-402Article Link
2. Mol Genet Metab. et al.(2008) Epub 95: 74