Recombinant Human CXCL5

CXCL5 is an inflammatory chemokine that belongs to the CXC chemokine family¹ that has a role in the development of cardiovascular diseases.² CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils.¹ It is expressed by epithelial cells within colorectal mucosa is a chemoattractant for neutrophils and has been implicated in Crohn's disease and ulcerative colitis.³ CXCL5 is important in growth and development of colorectal cancer, implicating a future role in both cancer therapy and diagnosis.⁴

The biological activity of Human ENA-78, 5-78 a.a. was determined by its ability to induce myeloperoxidase release from cytochalasin B treated human neutrophils (Schröder, J. et al., 1987, J. Immunol. 139:3474) or chemotaxis of mouse BaF/3 cells transfected with human CXCR-2. The expected ED₅₀ for these effects are typically 0.5 - 1.5 μg/ml or 0.3 - 1.5 ng/ml, respectively.

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Ala41

Lyophilized

The predicted molecular weight of Recombinant Human CXCL5 is Mr 8 kDa.

8

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Rajashekhar, G. et al. (2007) Physiol Genomics 31: 104
2. Mahsa, M. et al. (2008) Molec Diagnosis & Therapy 12: 391
3. Wågsäter, D. et al. (2007) International J Oncol. 31: 97
4. Nagelkerke, F. et al. (2008) Clinical Cancer Research 14: 2276