Recombinant Human FGF-20

Fibroblast growth factor 20 (FGF-20) is a secreted heparin binding growth factor and member of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation.¹ FGF-20 is most similar to FGF-9 and FGF-16. It signals through a variety of FGF receptors and is known to bind to heparin. FGF-20 is expressed in normal brain, particularly the cerebellum, and in some cancer cell lines. It is expressed preferentially in the substantia nigra pars compacta of the rat brain.² Expression of FGF-20 and DKK-1 is regulated by β-catenin during development and tumorigenisis, implying that FGF-20 may play a role in the oncogenisis induced by the Wnt signaling pathway.³ In NIH 3T3 cells, FGF-20 expression causes a transformed phenotype and these transformed cells are tumorigenic in nude mice.⁴ Recombinant rat FGF-20 is one of the neurotrophic factors, which enhances the survival of midbrain dopaminergic neurons in vitro. It has been demonstrated to possess therapeutic activity in two experimental models of intestinal inflammation.⁴

The biological activity of Human FGF-20 was determined by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts. The expected ED₅₀ for this effect is typically 0.2 - 1 ng/ml in the presence of 1 μg/ml of heparin.

Histidine Tag

hhhhhhapla evggflggle glgqqvgshf llppagerpp llgerrsaae rsarggpgaa qlahlhgilr rrqlycrtgf hlqilpdgsv qgtrqdhslf gilefisvav glvsirgvds glylgmndkg elygseklts ecifreqfee nwyntyssni ykhgdtgrry fvalnkdgtp rdgarskrhq kfthflprpv dpervpelyk dllmyt

Met

Lyophilized

The predicted molecular weight of Recombinant Human FGF-20 is Mr 24 kDa.

24

This recombinant protein was lyophilized from a 0.2 μm filtered solution in MOPS, ammonium sulphate ((NH₄)₂SO₄), ethylenediaminetetraacetic acid (EDTA), and dithiothreitol (DTT).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Katoh, M. et al. (2000) Biochem. Biophy. Res. Comm. 274:337
2. Ohmachi, S. et al. (2000) Biochem. Biophy. Res. Comm. 277:355
3. Chamorro, MN. et al. (2005) EMBO J. 24:73
4. Jeffers, M. et al. (2001) Cancer Res. 61:3131