Recombinant Human GM-CSF

Granulocyte-Macrophage Colony Stimulating Factor is a 22 kD, pleiotropic cytokine that is a white blood cell growth factor. It controls the production and function of blood cells by stimulating stem cells to produce granulocytes and monocytes. GM-CSF differs from G-CSF in that it affects more cell types including macrophages and eosinophils. Moreover, GM-CSF is part of the immune/inflammatory cascade, a process crucial for fighting infection. Interestingly, GM-CSF expression may have pathological implications. Autocrine expression of GM-CSF in myeloid leukemia cells is suspected to play a role in neoplasia, the formation of a new and abnormal growth of tissue. Additionally, GM-CSF expression has also been documented in certain solid tumors. There have also been reports of GM-CSF in synovial fluid from patients with arthritis suggesting that GM-CSF may play a role in tissue damage associated with the inflammatory process. Blocking GM-CSF is thought to have therapeutic potential by reducing inflammation. Some drugs are currently being developed to block GM-CSF.

The biological activity of Human GM-CSF was determined by a cell proliferation assay using the factor-dependent cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 343). The expected ED₅₀ for this effect is typically 6 - 30 pg/ml.

CAA26822

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Ala18

Lyophilized

The predicted molecular weight of Recombinant Human GM-CSF is Mr 14 kDa. Additionally, the actual molecular weight as observed by migration on SDS-PAGE is 14 kDa (reducing conditions).

14

This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.

This protein is stable for up to twelve months when stored at -20°C to -70°C. After thawing and addition of a carrier protein, this protein may be stored at 2°C to 8°C for one month or for long-term storage, aliquot and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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