Recombinant Human GCP-2

Chemokine (C-X-C motif) ligand 6 (CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as granulocyte chemotactic protein 2 (GCP-2). As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes.^1,2 It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2.² CXCL6 possesses antibacterial activity against Gram-positive and Gram-negative pathogenic bacteria of relevance to mucosal infections. CXCL6 causes membrane disruption in a dose-dependent manner. This is reduced in the presence of NaCl, which inhibits the binding of the peptide to the bacterial surface.³ CXCL6, in addition to its chemotactic and angiogenic properties, is likely to contribute to direct antibacterial activity during localized infection and thus plays a role in innate immunity.

The biological activity of Human GCP-2 was determined by its ability to induce myeloperoxidase release from cytochalasin-B treated human neutrophils (Schröder, J.M., et al. 1987, J. Immunol. 139:3474) or to induce chemotaxis of mouse BaF/3 cells transfected with hCXCR2. The expected ED₅₀ for these effects are typically 0.5 - 1.5 μg/ml and 3 - 15 ng/ml, respectively.

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Val40

Lyophilized

The predicted molecular weight of Recombinant Human GCP-2 is Mr 8 kDa.

8

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Van Damme, J. et al. (1993)Biochemistry 32: 10170
2. Van Damme, J. et al. (1997)Biochemistry 36: 2716
3. Linge, HM. et al. (2008)Antimicrobial Agents And Chemotherapy 52: 2599