Recombinant Human IL-17F

IL-17F is a homodimeric protein that is a member of the IL17 family of cytokines produced by activated T-cells and monocytes.¹ IL-17F is expressed by activated T cells and can stimulate production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover.² IL-17F is an important regulator of inflammatory responses that function differently than IL-17 in immune responses and diseases.³

The biological activity of Human IL-17F was determined by its ability to bind mIL-17 RC in a functional ELISA with an estimated KD < 3 nM.

The DNA sequence encoding the mature mouse IL-17F (Arg 21 - Ala 153) was inserted into a suitable expression vector and was expressed in E. coli (Hurst, S.D. et al., 2002, J. Immunol. 169(1):443 - 453).

Lyophilized

The predicted molecular weight of Recombinant Human IL-17F is Mr 15 kDa.

15

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Starnes T. et al. (2001) J. of Immonol. 167: 4137 2. Starovasnik MA. et al. (2001) Embo J. 20: 5332 3. Chen Dong et al. (2008) J Experimental Med. 205: 1063