Recombinant Human CXCL10

CXCL10, also known as IP-10 and CRG-2, is a small cytokine belonging to the CXC chemokine family. Binding of this protein to the cell surface chemokine receptor CXCR3, results in pleiotropic effects, including stimulation of monocytes, natural killer and T cell migration and modulation of adhesion molecule expression. CXCL10 is secreted by several cell types in response to IFN-γ including monocytes, endothelial cells and fibroblasts (1). CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity and inhibition of bone marrow colony formation and angiogenesis (2-3). Additionally, it is angiostatic and mitogenic for vascular smooth muscle cells. CXCL10 plays a role in the regulation of tubular cell proliferation following renal ischemia-reperfusion injury (4). CXCL10 shows anti-microbial and anti-bacterial activity (5-6).

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3. The ED₅₀ for this effect is 0.03‑0.18 µg/mL.

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Met

Lyophilized

The predicted molecular weight of Recombinant Human CXCL10 is Mr 8.7 kDa.

8.7

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Luster, AD. et al.(1985) Nature 315: 672
2. Dufour, JH. et al.(2002) J. Immunol. 168
3. Angiolillo, AL. et al.(1995) J. Exp. Med. 182: 155
4. Kaneko, S. et al.(2008) Nephron Exp. Nephrol. 109:(1):c29-38