Anti-Mouse IGF-II (Clone 122404)
Anti-Mouse IGF-II (Clone 122404)
Product No.: I-710
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Clone 122404 Target IGF-II Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Somatamedin A, Somatomedin C, C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974, Insulin-Like Growth Factor II Isotype IgG2a Applications ELISA Cap , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse IGF-II (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830831 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-800 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-586. Western Blotting: To detect Mouse IGF-II this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IGF-II is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-586. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rat Anti-Mouse Insulin-Like Growth Factor II (IGF-II) (Clone 122404) recognizes an epitope on Mouse IGF-II. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Insulin-like growth factor 2 (IGF-2) is a polypeptide hormone with structural and functional homology with IGF-I and pro-insulin.1 IGF-2 exerts its effects by binding to the IGF-1 receptor. IGF2 may also bind to the IGF-2 receptor. The major role of IGF2 is growth promoting hormone during gestation. IGF-2 has been shown to be an important regulator of fetoplacental growth.2 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Hoeflich, A. et al. (1994) Hormone Research 41: 66 2. Gruslin, A. et al.(2005) Proc Natl Acad Sci U S A. 102(31):11047-52. Technical Protocols  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
