Recombinant Mouse LIGHT

LIGHT is a type II transmemebrane protein and a member of the TNF cytokine family also referred to as TNFSF14 and it is identical with HVEM ligand. LIGHT is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells. LIGHT binds to three receptors; the herpes virus entry mediator type A receptor (HEVM), LTbR and a decoy receptor (DcR3).¹

The biological activity of Mouse LIGHT was determined by a cytotoxicity assay using HT29 cells, a human colon adenocarcinoma cell line (Zhai, Y. et al., 1998, J. Clin. Invest. 102:1142). The expected ED₅₀ for this effect is typically 2 - 5 μg/ml in the presence of 10 μg/ml of a cross-linking antibody PN:246 and 1 ng/ml of rhIFN-γ.

Histidine Tag

Q9QYH9

hhhhhhhhhh gggsgggsgg gsiegrdggk gswekliqdq rshqanpaah ltganaslig iggpllwetr lglaflrglt yhdgalvtme pgyyyvyskv qlsgvgcpqg langlpithg lykrtsrypk elellvsrrs pcgranssrv wwdssflggv vhleageevv vrvpgnrlvr prdgtrsyfg afmv

Met

Lyophilized

The predicted molecular weight of Recombinant Mouse LIGHT is Mr 21 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 24-34 kDa.

21

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with BSA as a carrier protein pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

1. Guo, Y. et al. (2005) Hybridoma (Larchmt). 24(6):309-13.