Recombinant Human M-CSF

Macrophage colony-stimulating factor (M-CSF), also known as CSF-1, is a secreted cytokine and key regulator of cellular proliferation, differentiation and survival of blood monocytes, tissue macrophages and their progenitor cells. It is produced by monocytes, fibroblasts and endothelial cells and binds to the CSF-1 receptor, also known as CD115. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis and secondary cytokine production in monocytes and macrophages (1). M-CSF also primes macrophage killing of tumor cells and microorganisms and stimulates pinocytosis. It is known as one of the factors essential for osteoclast development (2). M-CSF has been shown to play important roles in modulating dermal thickness and growth of the decidua and placenta during pregnancy (3). It is clinically used in the treatment of infection, malignancies and atherosclerosis, as well as, facilitating hematopoietic recovery after bone marrow transplantation (4).

The biological activity of Human M-CSF was determined in a cell proliferation assay using an M-CSF-dependent murine monocytic cell line, M-NFS-60 (Halenbeck, R. et al., 1989, Biotechnology 7:710 - 715). The expected ED₅₀ for this effect is 0.5 - 1.5 ng/ml.

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Solution

The predicted molecular weight of Recombinant Human M-CSF is Mr 18.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 37 kDa.

18.5

This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.

Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

Dry Ice

1. Nemunaitis, J. et al. (1998) Clin. Infect. Dis. 26:1279
2. Ko, JS. et al. (2002) Exp. Mol. Med. 34:340
3. Makrigiannakis, A. et al. (2006) Trends Endicrinol. Metab. 17:178
4. Van de Pol, CJ. et al. (1991) Biotechnol. Therap. 2:231