Recombinant Human CCL22/MDC

Chemokine (C-C motif) ligand 22, also known as CCL22 is secreted by dendritic cells and macrophages, and elicits its effects on its target cells by interacting with cell surface chemokine receptors such as CCR4.¹ CCL22 selectively chemo-attract T_h2 cytokine-producing cells^2,3 and acts as an important activator of eosinophils once these cells have migrated into tissue.⁴

The biological activity of Human MDC was determined by by its ability to chemoattract human T-lymphoblastoid CEM-NKR cells (Howell, D.N. et al., 1985, J. Immunol. 134:971 - 976) and its ability to chemoattract mouse BaF/3 cells transfected with hCCR4. The expected ED₅₀ for these effects are typically 3 - 9 ng/ml and 0.5 - 3 ng/ml, respectively.

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Gly25

Lyophilized

The predicted molecular weight of Recombinant Human CCL22 is Mr 8 kDa.

8

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Sozzani, S. et al. (2001) Eur. J. Immunol. 31:812
2. Elias, CG. et al. (1998) J Immunol. 161:5027
3. Gray, PW. et al. (1997) J Exp Med. 185:1604
4. Teixeira, MM. et al. (2003) J Leuko Biol. 73:356