Recombinant Mouse PDGF R-β

Beta-type platelet-derived growth factor receptor, also known as CD140b, PDGFRB and PDGF-R-beta, is a member of the protein kinase superfamily and CSF-1/PDGF receptor subfamily. The PDGF family consists of PDGF-A, -B, -C and -D, that form disulfide-linked homodimers and a heterodimer (1). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two tyroine kinase receptors (PDGF Rα and Rβ) (2). These two receptor isoforms dimerize upon binding PDGF leading to three possible receptor combinations, namely -αα, -ββ and -αβ. The extracellular region of the PDGF receptor-β consists of five immunoglobulin-like domains and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans leading to the activation of several intracellular pathways, such as MAPK, PI3K and PLCy (3). Many cell types, including fibroblasts and smooth muscle cells express both α and β receptors while others only express one (4).

The biological activity of Mouse PDGFR-β was determined by its ability to inhibit the PDGF-BB induced ³H-Thymidine incorporation in the NR6R-3T3 fibroblast cell-line (Raines, E.W.et al., 1985, Methods Enzymol. 109:749). The expected ED₅₀ for this effect is typically 0.02 – 0.05 μg/mL in the presence of 4 ng/mL rhPDGF-BB.

Fc Fusion Protein

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Leu32

Lyophilized

The predicted molecular weight of Recombinant Mouse PDGF Rβ is Mr 82.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 124 kDa.

82.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Bergsten, E. et al. (2001) Nat. Cell Biol. 3:512
2. Arcaro, A. et al. (2000) Mol. Cell. Biol. 20:3817
3. Chi, KD. et al. (1997) Oncogene 15:1051
4. Morerio, C. et al. (2004) Cancer Res. 64:264