Recombinant Mouse PIGF-2
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BackgroundPlacenta growth factor 2 (PlGF-2) is an isoform of PlGF1 and a member of the vascular endothelial growth factor (VEGF) family. PlGF-2 has heparin binding properties and Neuropilin-1 is a receptor for PlGF-2. Protein DetailsPurity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity The biological activity of Mouse PlGF-2 was determined by its ability to bind rmFlt-1/Fc in a functional ELISA. Immobilized rmFlt-1/Fc at 2 μg/ml (100 μL/well) can bind rmPlGF-2 with a linear range of 0.1 - 5 ng/ml. Protein Accession No. Amino Acid Sequence (als)agnn stevevvpfn evwgrsycrp meklvyilde ypdevshifs pscvllsrcs gccgdeglhc vpiktanitm qilkippnrd phfyvemtfs qdvlcecrpi lettkaerrk tkgkrkrsrn sqteephp N-terminal Sequence Analysis Ala24 & Ala27 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Mouse PlGF-2 is Mr 15.1 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 19-22 kDa. Predicted Molecular Mass 15.1 Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA). Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank References & Citations1. Neufeld, G. et al. (1998) J Biol Chem. 273: 22272 Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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