Recombinant Mouse TNF-α

TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

The biological activity of Mouse TNF-α was determined by by TNF mediated cytotoxicity in the mouse L-929 cell line, in the presence of the metabolic inhibitor actinomycin D (Matthews, N. et al., 1987. in Lymphokines and Interferons, a practical approach. M.J. Clemens, A.G. Morris and A.J.H Gearing, eds. IRL Press. p. 221). The expected ED₅₀ for this effect is typically 0.02 - 0.05 ng/ml.

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Met & Ser84

Lyophilized

The predicted molecular weight of Recombinant Mouse TNF-α is Mr 17 kDa. A small amount of recombinant protein lacking the N-terminal methionine and four additional amino acid residues is also present in the preparation.

17

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. El-Harith, EHA. et al. (2004) Saudi Med J. 25: 135
2. Adolf, GR. et al. (1990) Infec Immun. 58: 3996