Anti-Mouse TNFRSF19 – Biotin
Anti-Mouse TNFRSF19 – Biotin
Product No.: T502
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Target TNFRSF19 Product Type Polyclonal Antibody Alternate Names Tumor Necrosis Factor Receptor Superfamily, Member 19, TNFRSF19, Toxicity and JNK Inducer (TAJ), TAJ-Alpha, TRADE, TROY Applications ELISA Det , IHC FFPE , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse TNFRSF19 (Accession # Q9JLL3) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2832002 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse TNFRSF19 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse TNFRSF19 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T407. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN: as the capture antibody at 0.8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Mouse TNFRSF19. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:A106. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Mouse Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19) recognizes Mouse TNFRSF19. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Tumor necrosis factor receptor superfamily, member 19, also known as TNFRSF19 and TROY is a novel murine tumor necrosis factor receptor.1 It can be detected in most murine tissues examined, particularly in brain, reproductive organs, and late developmental stages of murine embryo, but not in tissues of the immune system.1 TNFRSF19 is a member of the TNF-receptor superfamily and is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Chen, L. et al. (1999) Genomics 62: 103
Technical ProtocolsELISA Det IHC FFPE  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
