Anti-8-oxo-Adenosine (8-oxoA) DNA/RNA Damage Monoclonal Antibody
(Clone 6E4) (12503)
Anti-8-oxo-Adenosine (8-oxoA) DNA/RNA Damage Monoclonal Antibody
(Clone 6E4) (12503)
Product No.: 12503
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Clone 6E4 Target 8-oxoA Formats AvailableView All Product Type Monoclonal Antibody Alternate Names -8-oxoAdenine, -8-oxo-Adenosine, -8-oxoA, -8-hydroxyadenosine Isotype Mouse IgG1κ Applications ELISA |
Data
Selectivity and Specificity of 6E4 in RNA:* Binds 8-oxoA in single and double stranded RNA
* Recognizes 8-oxoA in RNA base mispairs with standard geometry
Selectivity and Specificity of 6E4 in DNA:* Binds 8-oxoA in single and double stranded DNA
* Recognizes 8-oxoA in all DNA base mispairs
Clone 6E4 8-oxoA Specificity Determined by a competitive inhibition ELISA with nucleoside variants. The % B/Bo is plotted as a function of the concentration (nM) of the indicated competitor. 100% B/Bo indicates no inhibition by the indicated competitor.
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen 8-OxoA conjugated to KLH Product Concentration Lot Specific Formulation PBS, pH 7.4 State of Matter Liquid Product Preparation Protein G Affinity Chromatography Storage and Handling Store antibody at -20°C to -80°C. Avoid multiple freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Anti-8-oxoA is a mouse monoclonal antibody targeting oxidative damage in RNA, DNA, ATP and other nucleotide precursors and degradation products. Background This target is a byproduct of oxidative damage to the adenosine nucleobase in DNA, RNA, and nucleotides such as ATP. In DNA, 8-oxoA mispairs may reduce fidelity of replication and cause transcriptional errors by RNA pol II. In RNA, 8-oxoA damage
leads to attenuation of mRNA translation. Specialized base excision repair machinery and nucleotide sanitation processes are present to reduce the mutagenic consequences of 8-oxoA.
This antibody is the intellectual property of CymbaX, sequencing of this antibody is prohibited. Research Area DNA/RNA Damage References & CitationsDaniel J. Tew, Jakob M. Hebert, Brad J. Schmier, Discovery and properties of a monoclonal antibody targeting 8-oxoA, an oxidized adenine lesion in DNA and RNA, Redox Biology 62 (2023) 102658, PMC10074937 Technical Protocols Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
