Anti-Acetylated Lysine Antibody (56455)

Anti-Acetylated Lysine Antibody (56455)

Product No.: 56455


Clone 7F8 Target Acetylated Lysine Formats AvailableView All Product Type Monoclonal Isotype Mouse IgG1 Applications ELISA , IHC , IP , WB , ICC/IF


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Data

Immunohistochemistry analysis using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8 (56455). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody (56455) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.

Western Blot analysis of acetylated lysine showing detection of Acetylated Lysine protein using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8 (56455). Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody (56455) at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker.


Antibody Details Product Details Reactive Species All Host Species Mouse Immunogen Acetylated KLH Product Concentration Lot Specific Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1ug/mL. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Acetylated chicken erythrocyte histones. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity This antibody recognizes proteins containing acetylated lysine residues. It does not react with non-acetylated lysine residues. Background Acetylation is a common post-translational modification of lysine. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs). Post- translational acetylation and deacetylation of lysine have been recognized as intracellular signals that are critical in regulating gene transcription, cell cycle progression, apoptosis, DNA repair, and cytoskeletal organization. Regulation of protein acetylation is impaired in some cancers and polyglutamine diseases, and HDACs are promising targets for anti-cancer drugs currently under investigation. Research Area Neuroscience References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols ICC/IF Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

All

Host Species

Mouse

Immunogen

Acetylated KLH

Product Concentration

Lot Specific

Formulation

PBS, pH 7.4.

State of Matter

Liquid

Product Preparation

Purified by Protein G affinity chromatography

Storage and Handling

This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.

Regulatory Status

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Immunoblotting: use at 1ug/mL.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Acetylated chicken erythrocyte histones.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This antibody recognizes proteins containing acetylated lysine residues. It does not react with non-acetylated lysine residues.

Background

Acetylation is a common post-translational modification of lysine. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs). Post- translational acetylation and deacetylation of lysine have been recognized as intracellular signals that are critical in regulating gene transcription, cell cycle progression, apoptosis, DNA repair, and cytoskeletal organization. Regulation of protein acetylation is impaired in some cancers and polyglutamine diseases, and HDACs are promising targets for anti-cancer drugs currently under investigation.

Research Area

Neuroscience