Anti-Anthrax Lethal Factor (CT)

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Anthrax Lethal Factor (Anthrax LF) recognizes an epitope near the C-terminus of Anthrax LF. This polyclonal antibody was purified using affinity chromatography.

Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF)^1,2. PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR)³ and capillary morphogenesis protein 2 (CMG2),⁴ although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm. These toxins are usually sufficient to cause rapid cell death, and often the death of the organism.

1. Schwartz, M. N. (2001) New Engl. J. Med. 345:1621 2. Moayeri, M. et al. (2004) Curr. Opin. Microbiol. 7:19 3. Bradley, K. A. et al. (2001) Nature 414:225 4. Scobie, H. M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170