Anti-BAD (Phospho-Ser136) Antibody (43068)
Data
Immunohistochemistry: Detection of BAD (phospho-Ser136) in paraffin-embedded human breast carcinoma tissue.
Immunoblotting: Detection of BAD (phospho-Ser136) in extracts of
HT29 cells untreated or treated with UV. - -
- -
Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of Serine 136 (S-R-S(p)-A-P) derived from mouse BAD and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500- 1:1,000. A ban of ~23kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to BAD (Phospho-Ser136) Background The protein encoded by the BAD gene is a member of the Bcl-2 family whose members are regulators of programmed cell death. BAD protein positively regulates cell apoptosis by forming heterodimers with Bcl-xL and Bcl-2 and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin, were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. Antigen DetailsFunction Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. {ECO:0000250}. NCBI Gene Bank ID UniProt.org Research Area Phosphospecific Antibodies References & CitationsTechnical Protocols  |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
