Anti-CaMKII Antibody (56370)
Data
Immunoblotting: Use at 1-5ug/ml. A band of ~50-60 kDa is detected. Detection of CaMKII in mouse brain extract (Lane 2), rat brain extract (Lane 3), HeLa cell lysate (Lane 4), Hs-67 cell lysate (Lane 5).
Immunohistochemistry: use at 1-10ug/ml. Detection of CaMKII in human lung. - -
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Antibody DetailsProduct DetailsReactive Species Bovine ⋅ Chicken ⋅ Guinea Pig ⋅ Hamster ⋅ Human ⋅ Mouse ⋅ Rabbit ⋅ Rat Host Species Rabbit Immunogen Synthetic peptide corresponding to aa 7-20 (N-terminus) of rat CaMKII delta isoform conjugated to KLH. Product Concentration Lot Specific Formulation PBS, 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by protein A affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: Use at 1-5ug/mL. A band of ~50-60 kDa is detected.
Immunohistochemistry: use at 1-10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes alpha, delta, and gamma isoforms of human, mouse, rat, bovine, hamster, guinea pig, chicken, and rabbit CaMKII. Background Calmodulin-dependent protein kinase II (CaMKII) is a serine/threonine-specific protein kinase that is regulated by the Ca2+/calmodulin complex. CaMKII is involved in many signaling cascades and is thought to be an important mediator of learning and memory. CaMKII is also necessary for Ca2+ homeostasis and reuptake in cardiomyocytes, chloride transport in epithelia, positive T-cell selection, and CD8 T-cell activation. CaMKII has 28 different isoforms that derive from the alpha, beta, gamma, and delta genes. Misregulation of CaMKII is linked to Alzheimer's disease, Angelman syndrome, and heart arrhythmia. NCBI Gene Bank ID Research Area Enzymes References & CitationsTechnical Protocols  Certificate of Analysis |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
