Anti-CDC25a Antibody (13022)

Anti-CDC25a Antibody (13022)

Product No.: 13022

- -
- -
Target
CDC25a
Product Type
Polyclonal
Alternate Names
EC 3.1.3.48, Dual specificity phosphatase Cdc25A
Isotype
Whole IgG
Applications
IP
,
WB

- -
- -
Select Product Size

Data

<b>Detection of human CDC25a by western blot of immunoprecipitates.</b> <i>Samples:</i> Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. <i>Antibodies:</i> Affinity purified rabbit anti-CDC25a antibody 13022 (lot 13022-2) used for IP at 6 µg per reaction. CDC25a was also immunoprecipitated by rabbit anti-CDC25a antibody. For blotting immunoprecipitated CDC25a|13022 was used at 1 µg/ml. <i>Detection:</i> Chemiluminescence with an exposure time of 3 minutes.Detection of human CDC25a by western blot of immunoprecipitates. Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDC25a antibody 13022 (lot 13022-2) used for IP at 6 µg per reaction. CDC25a was also immunoprecipitated by rabbit anti-CDC25a antibody. For blotting immunoprecipitated CDC25a, 13022 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody 13022 (lot 13022-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- -
- -

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
Synthetic peptide representing a portion of the protein encoded by exon 7 (LocusLink ID 993).
Product Concentration
Lot Specific
Formulation
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
State of Matter
Liquid
Product Preparation
Purified by immunoaffinity chromatography
Storage and Handling
Store at 2 - 8°C. Antibody is stable at 2 - 8°C for 1 year.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: 0.1 - 1 ug/ml.

Immunoprecipitation: 1- 4 ug/mg of lysate.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit polyclonal antibody specific to human CDC25a, other species have not been tested.

Antigen Details

Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
NCBI Gene Bank ID
UniProt.org
Research Area
Enzymes

References & Citations

Immunoprecipitation Protocol
General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.