Anti-Digoxigenin Antibody (90012)

Anti-Digoxigenin Antibody (90012)

Product No.: 90012

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Clone
21H8
Target
Digoxigenin
Formats AvailableView All
Product Type
Monoclonal
Isotype
IgG1
Applications
ELISA
,
IHC
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactivity Species
Digoxigenin
Host Species
Mouse
Immunogen
Digoxigenin conjugate.
Formulation
Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
State of Matter
Lyophilized
Product Preparation
Purified by Protein A affinity chromatography
Storage and Handling
This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA: use at 0.1-1.0ug/ml

Immunoblotting:

User should use at 2-5ug/ml.

Immunohistochemistry: Use at 1-5ug/ml.

These are recommended concentrations.

End user should determine optimal concentrations for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Monoclonal Antibody specific to Digoxigenin
Background
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves). It is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein and is often used as an immunohistochemical marker for in situ hybridization. In this case it is conjugated to a single species of RNA nucleotide triphosphate (typically uridine), which is then incorporated into RNA as it is synthesized.

Antigen Details

Research Area
Hormones/Proteins

References & Citations

Indirect Elisa Protocol
IHC
General Western Blot Protocol

Formats Available

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Prod No.
Description
90012
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.