Anti-Endotoxin Antibody (15307)

Anti-Endotoxin Antibody (15307)

Product No.: 15307

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Clone
HEC10-172.5
Target
Endotoxin
Formats AvailableView All
Product Type
Monoclonal
Isotype
Mouse IgM
Applications
ELISA

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Select Product Size

Data

Reactivity KeyReactivity Key
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Antibody Details

Product Details

Reactive Species
E. Coli J5, K. Pneumoniae, S. Marcescens, P. Mirabilis, P. Vulgaris, Ps Aeruginosa
Host Species
Mouse
Immunogen
E. coli O111 B4 J5 cells
Product Concentration
Lot Specific
Formulation
This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added.
State of Matter
Liquid
Product Preparation
Antibodies are purified by a multi-step process including the use of PEG purification chromatography.
Storage and Handling
Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
These antibodies have been qualified for use in ELISA to detect gram-negative endotoxin and in dot-blots to detect cells that express gram negative endotoxin.

End users should determine optimal concentrations for their applications.

Customer feedback: #15305 was conjugated to colloidal gold and used successfully in a lateral flow assay format.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Monoclonal Antibody specific to Endotoxin (See Reactivity Key).
Background
Endotoxin is the lipopolysaccharide (LPS) complex associated with the outer membrane of Gram-negative bacteria such as Escherichia coli, Salmonella, Shigella, Pseudomonas, Neisseria, Haemophilus influenzae, Bordetella pertussis and Vibrio cholerae. Toxicity is associated with the lipid component (Lipid A) and immunogenicity is associated with the polysaccharide components. The cell wall antigens (O antigens) of Gram-negative bacteria are components of LPS. LPS elicits a variety of inflammatory responses, and it activates complement by the alternative (properdin) pathway.
Research Area
Infectious Disease

References & Citations

Indirect Elisa Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
15307
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.