Anti-Enterovirus 68, Capsid (Clone EV-D68-105) – Purified No Carrier Protein

Sequenced from PBMCs from a donor who had recovered from a naturally-occurring Enterovirus D68 infection.

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -80°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

Clone EV-D68-105 is a human IgG monoclonal antibody specifically binds to the capsid of Enterovirus D68 from clades B and D.

EV-D68 is a highly transmissible respiratory disease that primarily impacts children. It is among the 100-plus non-polio enteroviruses and is listed as a NIAID Biodefense Priority Pathogen. The respiratory condition can vary from mild (resembling a common cold) to more severe, potentially resulting in acute flaccid myelitis (AFM), a condition characterized by lasting muscle weakness. The majority of individuals with EV-D68 encounter mild symptoms and recuperate without significant complications¹. No vaccines are currently accessible for preventing EV-D68 infections, but practical measures can aid in lowering the chances of transmission. The virus is transmitted via respiratory secretions (saliva, nasal mucus, and sputum) when an infected individual coughs, sneezes or makes contact with surfaces subsequently touched by others².

Clone EV-D68-105 specifically binds to at least one of the VP proteins of the EV-D68 capsid. The capsid is made up of four viral proteins (VP1, VP2, VP3, and VP4) that are crucial for the virus's ability to bind and enter host cells. Clone EV-D68-48 clone effectively bind and neutralize the virus, specifically the clinically relevant clades B and D, preventing it from infecting cells and spreading⁴.

EV-D68 can be found in human respiratory epithelial cells, gastrointestinal cells, and motor neurons within the spinal cord's grey matter​.

1. Enterovirus D68 (EV-D68): Symptoms, Treatment & Prevention. Cleveland Clinic. Accessed July 13, 2024. https://my.clevelandclinic.org/health/diseases/21669-enterovirus-d68
2. CDC. About Enterovirus D68. Non-Polio Enterovirus. Published June 7, 2024. Accessed July 13, 2024. https://www.cdc.gov/non-polio-enterovirus/about/about-enterovirus-d68.html
3. Li X, Li Y, Fan S, et al. J Med Chem. 2022;65(21):14792-14808.
4. Vogt MR, Fu J, et al. Sci Immunol. 2020;5(49).