Anti-HGAL Antibody (34044)
Anti-HGAL Antibody (34044)
Product No.: 34044
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Clone 1H1-A7 Target HGAL Formats AvailableView All Product Type Monoclonal Alternate Names Germinal center B-cell-expressed transcript 2 protein, Germinal center-associated lymphoma protein, hGAL Isotype Mouse IgG2a Applications IHC , WB |
Data
Immunohistochemistry: use at 1-10ug/ml on paraffin-embedded tissues with heat-induced antigen retrieval in citrate buffer (10mM|pH 6.0
Immunoblotting: use at 1-10ug/ml.
HGAL mRNA and protein expression in lysates of indicated cell lines. Actin served as a loading control. - -
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen GST-HGAL fusion protein (Accession no. AF521911) Product Concentration Lot Specific Formulation This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added. State of Matter Liquid Product Preparation Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunohistochemistry: use at 1-10ug/ml on paraffin-embedded tissues with heat-induced antigen retrieval in citrate buffer (10mM, pH 6.0, 10 minutes). Immunoblotting: use at 1-10ug/mlHGAL mRNA and protein expression in lysates of indicated cell lines. Actin served as a loading control. These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human HGAL. Background The human germinal-center-associated lymphoma (HGAL) gene was originally identified from an expressed sequence tag that was associated with improved survival in patients with diffuse large B-cell lymphoma (DLBCL). Cloning and characterization of HGAL indicated that it is induced by IL-4. HGAL is a cytoplasmic protein and has been demonstrated in follicular lymphoma, Burkitt lymphoma, lymphocyte-predominant Hodgkin lymphoma, and a subset of DLBCL. HGAL expression is an important predictor of overall survival in patients with germinal center- derived tumors. HGAL protein is also found in germinal centers of normal tonsils and lymph nodes. Function Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation. {PubMed:17823310, PubMed:20844236, PubMed:23299888}. NCBI Gene Bank ID UniProt.org Research Area Cancer Research References & Citations1) Fouad-Younes S et al. 2010 Am J Surg Pathol 34: 1266-1276. 2) Natkunam Y et al. 2006 Blood 109: 298-305. 3) Natkunam Y et al. 2005 Blood 105: 3979-3986. 4) Lossos IS et al. 2003 Blood 101: 433-440. Technical Protocols  Certificate of Analysis |
Formats Available
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Prod No. | Description |
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H210 | |
H211 | |
H212 | |
H213 | |
H219 | |
H220 | |
H216 | |
H214 | |
H221 | |
34044 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
