Anti-Human Beclin-1 (NT)

PN:B483

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human Beclin-1 recognizes an epitope near the N-terminus domain of human, mouse and rat Beclin-1. This polyclonal antibody was purified using affinity chromatography.

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells.^1,2 Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6,^3,4 is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors.⁵ Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.⁶

1. Gozuacik D and Kimchi A. (2004) Oncogene. 23:2891-906.
2. Kisen, GO. et al. (1993) Carcinogenesis 14:2501-5.
3. Liang, XH. et al. (1998) J. Virol. 72:8586-96.
4. Kametaka, S. et al. (1998) J. Biol. Chem. 273:22284-91.