Anti-Human Caspase-3 – DyLight® 755

Purified Recombinant Human CASP3 (>98%)

This DyLight^® 755 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

This DyLight^® 755 conjugate is stable when stored at 2-8°C. Do not freeze.

Next Day 2-8°C

Mouse Anti-Human Caspase-3 Active Subunit (p17) (CASP3) (Clone C31.129) recognizes an epitope on Human CASP3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Caspase-3 is a member of the caspase family of cysteine proteases and plays a key role in mediating Fas (CD95) and tumor necrosis factor–1 (TNFR-1) induced apoptosis. Caspases can be divided into three groups: apoptotic initiators, apoptotic executioners, and inflammatory mediators.^1,2 Caspase-3 is activated during apoptotic signaling events by upstream proteases including caspase 6 and caspase 8.³ Caspase-3 is thought to be essential for chromatin margination, DNA fragmentation and nuclear collapse during apoptosis.³

1. Adadah, BA. et al. (2000) Journal of Neurotoma 17(10): 811
2. Salvesen, GS. et al. (1999) Proc. Natl. Acad. Sci. USA 96(20): 10964
3. Slee, EA. et al. (2001) J. Biol. Chem. 276(10):7320