Anti-Human CCL25 (Clone 52529)

Product No.: C1399


Clone 52529 Target CCL25 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Chemokine (C-C Motif) Ligand 25, SCYA25, TECK, Ckb15 Isotype IgG1 Applications ELISA Cap , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human CCL25 (Accession # AAB69981) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828456 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T185 at a concentration of approximately 200 ng/ml. A suggested standard for this assay is PN:T165. Western Blotting: To detect Human CCL25 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL25 is 0.1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human CCL25 (Clone 52529) recognizes an epitope on Human CCL25. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed ChemoKine). CCL25 is believed to play a role in the development of T-cells.¹ It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 is expressed in the small intestinal epithelium and plays an important role in mediating lymphocyte recruitment to this site.² CCL25 is also highly expressed in the thymus, where it binds to its receptor CCR9 on thymocytes promoting migration and activation.³ PubMed CCL25 Research Area Chemokine References & Citations 1. Zlotnik, A. et al. (1997) Immunity. 7(2):291-301. 2. Agace, W. et al. (2006) J Immunol. 176:3642 3. Teixidó, J. et al. (2007) J Leukoc Biol. 82:380 View product citations for antibody C1399 on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human CCL25 (Accession # AAB69981)

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828456

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T185 at a concentration of approximately 200 ng/ml. A suggested standard for this assay is PN:T165.
Western Blotting: To detect Human CCL25 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL25 is 0.1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human CCL25 (Clone 52529) recognizes an epitope on Human CCL25. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed ChemoKine). CCL25 is believed to play a role in the development of T-cells.¹ It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 is expressed in the small intestinal epithelium and plays an important role in mediating lymphocyte recruitment to this site.² CCL25 is also highly expressed in the thymus, where it binds to its receptor CCR9 on thymocytes promoting migration and activation.³

PubMed

CCL25

Research Area

Chemokine

References & Citations

1. Zlotnik, A. et al. (1997) Immunity. 7(2):291-301.
2. Agace, W. et al. (2006) J Immunol. 176:3642
3. Teixidó, J. et al. (2007) J Leukoc Biol. 82:380

View product citations for antibody C1399 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
C1428	Anti-Mouse CCL25 (Clone 89818)
C1399	Anti-Human CCL25 (Clone 52529)
C1429	Anti-Mouse CCL25 (Clone 89827)
T396	Recombinant Mouse TECK
T491	Anti-Mouse CCL25-Biotin
T185	Anti-Human CCL25-Biotin

Formats Available

Prod No.	Description
C1399	Anti-Human CCL25 (Clone 52529)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human CCL25 (Clone 52529)