Anti-Human CCL28 (Clone 62705)
Anti-Human CCL28 (Clone 62705)
Product No.: C1451
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Clone 62705 Target CCL28 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Chemokine (C-C Motif) Ligand 28, SCYA28, MEC, CCK1 Isotype IgG1 Applications N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen E. coli - Derived Recombinant Human CCL28 (Accession # Q9NRJ3) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828514 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human CCL28 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for rhCCL28 is approximately 20 ng/lane and 50 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human CCL28 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CCL28 (at 4 ng/ml) is 15 - 45 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human CCL28 (Clone 62705) recognizes an epitope on Human CCL28. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3.1,2,3 CCL28 is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury.3 CCL28 is a key regulator of IgA antibody-secreting cells (ASCs) accumulation in the mammary gland and thus controls the passive transfer of IgA antibodies from mother to infant.4 PubMed NCBI Gene Bank ID UniProt.org Research Area Chemokine References & Citations1. Kunkel, SL. et al. (2003) J Clin. Invest. 111: 1001 2. John, AE. et al. (2005) J. Pathol. 166(2): 345 3. Hieshima, GB. et al. (2003) J Immunol. 170: 1452 4. Butcher, EC. et al. (2004) J Exp. Med. 200: 805 Technical ProtocolsN  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
