Anti-Human CD107a (LAMP-1) – Purified No Carrier Protein

Human adult adherent peripheral blood cells

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

H4A3 activity is directed against human LAMP-1 (CD107a).

Lysosomes are membrane-bound vacuoles that digest foreign materials and perform specialized autolysis functions¹. LAMP-1 is a lysosome-associated membrane glycoprotein¹ involved in lysosomal biogenesis, pH regulation, autophagy, and cholesterol homeostasis that also plays a role in natural killer (NK) cell cytotoxicity and protects NK cells from degranulation-associated damage induced by their own cytotoxic granule content². Additionally, LAMP-1 presents carbohydrate ligands to selectins and facilitates microbial infection of Lassa virus and Mumps virus via interactions with viral glycoprotein and fusion protein F, respectively. LAMP-1 is highly abundant in lysosomes and can be used as a maker to determine the amount and distribution of lysosomes³.

H4A3 was generated by immunizing Sprague Dawley rats with NIH/3T3 cells⁴. Spleen cells were fused with murine myeloma P3X653-Ag8 cells. The antibody was deposited in the Developmental Studies Hybridoma Bank when first developed.

LAMP-1 primarily localizes to the periphery of the lysosome and is a constituent of the lysosome membrane. LAMP1 is ubiquitously expressed in human tissues.

Galaptin

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