Anti-Human CD107a (LAMP-1) – PE
Anti-Human CD107a (LAMP-1) – PE
Product No.: C2525
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Clone H4A3 Target CD107a Formats AvailableView All Product Type Hybridoma Monoclonal Antibody
Alternate Names LGP-120 Isotype Mouse IgG1 κ Applications IF , IHC , IP , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Human adult adherent peripheral blood cells Product Concentration 0.2 mg/ml Formulation This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. State of Matter Liquid Storage and Handling This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Excitation Laser Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm) Additional Applications Reported In Literature ? IHC, IF, WB, IP, Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity H4A3 activity is directed against human LAMP-1 (CD107a).
Background Lysosomes are membrane-bound vacuoles that digest foreign materials and perform specialized autolysis functions1. LAMP-1 is a lysosome-associated membrane glycoprotein1 involved in lysosomal biogenesis, pH regulation, autophagy, and cholesterol homeostasis that also plays a role in natural killer (NK) cell cytotoxicity and protects NK cells from degranulation-associated damage induced by their own cytotoxic granule content2. Additionally, LAMP-1 presents carbohydrate ligands to selectins and facilitates microbial infection of Lassa virus and Mumps virus via interactions with viral glycoprotein and fusion protein F, respectively. LAMP-1 is highly abundant in lysosomes and can be used as a maker to determine the amount and distribution of lysosomes3. H4A3 was generated by immunizing Sprague Dawley rats with NIH/3T3 cells4. Spleen cells were fused with murine myeloma P3X653-Ag8 cells. The antibody was deposited in the Developmental Studies Hybridoma Bank when first developed. Antigen Distribution LAMP-1 primarily localizes to the periphery of the lysosome and is a
constituent of the lysosome membrane. LAMP1 is ubiquitously expressed in human tissues.
Ligand/Receptor Galaptin NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Immunology . Neuroscience References & Citations1 Chen JW, Murphy TL, Willingham MC, et al. J Cell Biol. 101(1):85-95. 1985. 2 https://www.uniprot.org/uniprotkb/P11279/entry 3 Jensen SS, Aaberg-Jessen C, Christensen KG, et al. Int J Clin Exp Pathol. 6(7):1294-1305. 2013. 4 Hughes EN, August JT. J Biol Chem. 256(2):664-671. 1981. 5 Piccolo P, Sabatino V, Mithbaokar P, et al. Mol Genet Genomic Med. 7(9):e844. 2019. 6 Yu T, Chung C, Shen D, et al. Hum Mol Genet. 21(14):3205-3214. 2012. 7 Sudhan DR, Siemann DW. Clin Exp Metastasis. 30(7):891-902. 2013. 8 Faron M, Fletcher JR, Rasmussen JA, et al. Infect Immun. 81(8):2800-2811. 2013. 9 Fernandes MC, Flannery AR, Andrews N, et al. Cell Microbiol. 15(6):977-991. 2013. 10 Chapel A, Kieffer-Jaquinod S, Sagné C, et al. Mol Cell Proteomics. 12(6):1572-1588. 2013. 11 Del Giudice MG, Ugalde JE, Czibener C. Infect Immun. 81(3):956-964. 2013. 12 Roset MS, García Fernández L, DelVecchio VG, et al. Infect Immun. 81(2):521-530. 2013. 13 Notte A, Ninane N, Arnould T, et al. Cell Death Dis. 4(5):e638. 2013. 14 McDonough JA, Newton HJ, Klum S, et al. mBio. 4(1):e00606-12. 2013. 15 Newton HJ, Kohler LJ, McDonough JA, et al. PLoS Pathog. 10(7):e1004286. 2014. 16 Kohler LJ, Reed ShC, Sarraf SA, et al. mBio. 7(4):e01127-16. 2016. 17 Byndloss MX, Tsai AY, Walker GT, et al. mBio. 10(4):e01538-19. 2019. 18 Chong A, Starr T, Finn CE, et al. Mol Microbiol. 112(4):1270-1283. 2019. 19 Yang Z, Funke BH, Cripe LH, et al. Circ Cardiovasc Genet. 3(2):129-137. 2010. 20 Torres AG, Fabani MM, Vigorito E, et al. Nucleic Acids Res. 40(5):2152-2167. 2012. 21 Bekier ME 2nd, Wang L, Li J, et al. Mol Biol Cell. 28(21):2833-2842. 2017. Technical ProtocolsCertificate of Analysis |
Formats Available
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Prod No. | Description |
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C2521 | |
C2527 | |
C2522 | |
C2523 | |
C2524 | |
C2525 | |
C2526 |
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