Anti-Human CD16 (Clone 3G8) – DyLight® 488
Anti-Human CD16 (Clone 3G8) – DyLight® 488
Product No.: C6856
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Clone 3G8 Target CD16 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names FcγRIII, Fc Gamma RIII Isotype IgG1 κ Applications FC |
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Antibody DetailsProduct DetailsReactive Species Baboon ⋅ Chimpanzee ⋅ Cynomolgus Monkey ⋅ Marmoset ⋅ Pigtailed Macaque ⋅ Rhesus Monkey ⋅ Squirrel Monkey ⋅ Human Host Species Mouse Immunogen Human PMN cells Product Concentration 0.2 mg/ml Formulation This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C Excitation Laser Blue Laser (493 nm) RRIDAB_2893586 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 3G8 antibody for staining cells in flow cytometry is ≤ 5.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Reported Applications For Relevant Conjugates ? B CyTOF® CODEX IHC (Frozen) IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 3G8 recognizes an epitope on human CD16. This clone also cross-reacts with non-human primate CD16.
Background CD16 antibody, 3G8, recognizes human CD16, also known as low-affinity IgG receptor III (FcγRIII). There are two distinct forms of CD16, CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a 50-65 kDa heterooligomeric polypeptide-anchored transmembrane protein expressed by NK cells, macrophages, and subsets of monocytes1. CD16b is a 48 kDa monomeric glycosylphosphatidylinositol (GPI)-anchored protein expressed on neutrophils1. Upon binding to the Fc portion of IgG or IgG-antigen complex, both CD16 isoforms induce signaling cascades resulting in multiple functions, including phagocytosis, cytokine release, proliferation, degranulation, and antibody dependent cell-mediated cytotoxicity (ADCC)2.
Antigen Distribution CD16 is expressed on NK cells, activated monocytes, macrophages, neutrophils, and placental trophoblasts.
Ligand/Receptor Aggregated IgG, IgG-antigen complex Function Low affinity IgG Fc receptor, phagocytosis, ADCC NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity References & Citations1. Ravetch JV & Perussia B. (1989) J Exp Med. 170(2):481-497 2. Nimmerjahn F & Ravetch JV (2008) Nat Rev Immunol. 8(1):34-47 Technical Protocols Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
