Anti-Human CD16 (Clone 3G8) – Purified

Human PMN cells

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This purified antibody is stable when stored at 2-8°C. Do not freeze.

Next Day 2-8°C

CyTOF®
IHC (Frozen)
IP
B

Clone 3G8 recognizes an epitope on human CD16. This clone also cross-reacts with non-human primate CD16.

CD16 antibody, 3G8, recognizes human CD16, also known as low-affinity IgG receptor III (FcγRIII). There are two distinct forms of CD16, CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a 50-65 kDa heterooligomeric polypeptide-anchored transmembrane protein expressed by NK cells, macrophages, and subsets of monocytes¹. CD16b is a 48 kDa monomeric glycosylphosphatidylinositol (GPI)-anchored protein expressed on neutrophils¹. Upon binding to the Fc portion of IgG or IgG-antigen complex, both CD16 isoforms induce signaling cascades resulting in multiple functions, including phagocytosis, cytokine release, proliferation, degranulation, and antibody dependent cell-mediated cytotoxicity (ADCC)².

CD16 is expressed on NK cells, activated monocytes, macrophages, neutrophils, and placental trophoblasts.

Aggregated IgG, IgG-antigen complex

Low affinity IgG Fc receptor, phagocytosis, ADCC

1. Ravetch JV & Perussia B. (1989) J Exp Med. 170(2):481-497
2. Nimmerjahn F & Ravetch JV (2008) Nat Rev Immunol. 8(1):34-47