Anti-Human CD166 (ALCAM) (Clone 3A6) – Purified in vivo PLATINUMTM Functional Grade
Anti-Human CD166 (ALCAM) (Clone 3A6) – Purified in vivo PLATINUMTM Functional Grade
Product No.: C712
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Clone 3A6 Target CD166 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD6 ligand, Activated Leukocyte Cell Adhesion Molecule (ALCAM) Isotype IgG1 κ Applications FC , IF , IF Microscopy , IHC , IP |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Cultured human thymic epithelial cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Applications and Recommended Usage? Quality Tested by Leinco FC Additional Applications Reported In Literature ? IHC, IP, IF, IF Microscopy Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity 3A6 activity is directed against human CD166 (ALCAM) and cross-reacts with ovine tissues. Background Activated leukocyte cell adhesion molecule (ALCAM) is a member of the immunoglobulin superfamily and a cell surface glycoprotein1. In normal physiology, ALCAM functions in cell adhesion, is known to promote T cell activation and proliferation by interacting with CD6, and functions in angiogenesis, monocyte transmigration, leukocyte intravasation across the blood-brain barrier, hematopoiesis, neurite extension, osteogenesis, and embryonic implantation in the uterus. In cancer, ALCAM is a prognostic marker of disease progression and acts as a modulator of progression by controlling cell proliferation, adhesion, migration, and invasion.
ALCAM participates in homophilic ALCAM-ALCAM interactions as well as numerous heterophilic interactions1. Ligands include CD6, galectin-8, endophilin-A3/galectin-8, CD9, S100B, and ezrin. Additionally, SOSTDC1 is a novel ligand of ALCAM that promotes invasion and facilitates liver metastasis in colorectal cancer through activation of the Src-P13K/AKT pathways2. ALCAM is a type I transmembrane molecule with a large glycosylated extracellular domain1. Two isoforms have been confirmed at the protein level: ALCAM-Iso1, which is the full length isoform, and ALCAM-Iso2, which lacks exon 13. ALCAM is proteolytically cleaved at its extracellular domain by the transmembrane metalloprotease ADAM17, with ALCAM-Iso2 more susceptible to cleavage. 3A6 was produced by immunizing mice with human thymic epithelial cells and then fusing spleen cells with P3X63Ag8 myeloma cells3. 3A6 cross reacts with ovine mesenchymal stromal cells from iliac crest bone marrow aspirates4. Antigen Distribution CD166 is expressed on neurons, activated leukocytes, hematopoietic stem cells, mesenchymal stem cells, bone marrow stromal cells, activated T cells, activated B cells, activated monocytes, thymic epithelial cells, vascular endothelial cells, fibroblasts, keratinocytes, myeloid progenitors, tumor cells, and cancer stem cells. NCBI Gene Bank ID Research Area Cell Adhesion . Cell Biology . Immunology . Neuroscience . Synaptic Biology References & Citations1. Ferragut F, Vachetta VS, Troncoso MF, et al. Cytokine Growth Factor Rev. 61:27-37. 2021.
2. Bartolomé RA, Pintado-Berninches L, Jaén M, et al. Oncogene. 39(38):6085-6098. 2020. 3. Patel DD, Fong AM, Mann KP, et al. CD166 Workshop: Tissue distribution and functional analysis of antibodies reactive for CD166, a ligand for CD6. In: Kishimoto T, editor. Leukocyte Typing IV. Oxford: Oxford University Press; 1997. P.461-464. 4. Sanjurjo-Rodríguez C, Castro-Viñuelas R, Hermida-Gómez T, et al. PLoS One. 12(1):e0171231. 2017. 5. Piazza T, Cha E, Bongarzone I, et al. J Cell Sci. 118(Pt 7):1515-1525. 2005. 6. Tondreau T, Dejeneffe M, Meuleman N, et al. BMC Genomics. 9:166. 2008. 7. Srouji S, Kizhner T, Ben David D, et al. Calcif Tissue Int. 84(2):138-145. 2009. 8. Katsube Y, Kotobuki N, Tadokoro M, et al. Gene Ther. 17(4):494-502. 2010. 9. Brune JC, Tormin A, Johansson MC, et al. Int J Cancer. 129(2):319-330. 2011. 10. Ali H, Al-Yatama MK, Abu-Farha M, et al. PLoS One. 10(4):e0122465. 2015. 11. Prins HJ, Schulten EA, Ten Bruggenkate CM, et al. Stem Cells Transl Med. 5(10):1362-1374. 2016. 12. Fridriksdottir AJ, Kim J, Villadsen R, et al. Nat Commun. 6:8786. 2015. 13. Gong B, Zheng L, Lu Z, et al. Mol Med Rep. 23(1):43. 2021. 14. Yeh SP, Chang JG, Lin CL, et al. Leukemia. 19(8):1505-1507. 2005. 15. Levesque MC, Heinly CS, Whichard LP, et al. Arthritis Rheum. 41(12):2221-2229. 1998. 16. Ishiguro F, Murakami H, Mizuno T, et al. J Thorac Oncol. 7(5):890-899. 2012. 17. Bhattacharya S, Mathew G, Ruban E, et al. J Proteome Res. 9(12):6112-6125. 2010. Technical ProtocolsCertificate of Analysis |
Formats Available
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Prod No. | Description |
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C713 | |
C714 | |
C718 | |
C719 | |
C721 | |
C722 | |
C723 | |
C720 | |
C710 | |
C712 |
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