Anti-Human CD194 (CCR4) (Mogamulizumab)

Humanization of mouse anti-CCR4 mAb7.

This biosimilar antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Recombinant biosimilar antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Functional grade biosimilar antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only (RUO). Non-Therapeutic.

2-8°C Wet Ice

This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Mogamulizumab. Clone KW-0761 recognizes human CD194 (CCR4). This product is for research use only.

Clone KW-0761 (Mogamulizumab) is a research-grade, afucosylated, humanized monoclonal antibody generated from mouse anti-CCR4 mAb7 that targets human CCR4.³ CC chemokine receptor type 4 (CCR4) is a protein that belongs to the G protein-coupled receptor family and is a receptor for a variety of CC chemokines including MCP-1, MIP-1, RANTES, TARC, and Macrophage-derived chemokine. Chemokines are involved in the development, homeostasis, and function of the immune system and are known to regulate cell trafficking of various types of leukocytes. In a 2018 Phase I clinical trial, Mogamulizumab was found to decrease the number of HTLV-1–infected cells and the levels of inflammatory markers related to HTLV-1–Associated Myelopathy.⁵

CCR4 is expressed on a variety of cell types: T lymphocytes (Th2, Th17, and regulatory T cells), platelets, NK cells, monocytes, macrophages, dendritic cells, neurons, microglia, and astroglia.¹ Expression of CCR4 is increased on leukemic cells in cutaneous T-cell lymphoma (CTCL).²

CCL17 and CCL22

1. Nicolay, J. et al. (2021) Eur J Immunol. 51(7):1660-1671.

2. Bogacka, J. et al. (2022) Int J Mol Sci.. 23(24):15638.

3. Yamamoto, K. et al. (2010) J Clin Oncol. 28(9):1591-8.

4. Mimura, Y. et al. (2018) Protein Cell 9(1):47-62.

5. Yamano, Y. et al. (2018) N Engl J Med 378 (6), 529-538.