Anti-Human CD22 – Biotin
Anti-Human CD22 – Biotin
Product No.: C180
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Clone RFB-4 Target CD22 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names BL-CAM, MGC130020, SIGLEC2 Isotype IgG1 Applications FC |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Product Concentration 0.5 mg/ml Formulation This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This biotinylated antibody is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828867 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this RFB-4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human CD22 (Clone RFB-4) recognizes Human CD22. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD22 recognizes the (Mr 135 kDa) cytoplasmic antigen of all B-lymphocytes. The CD22 antigen is only expressed on the cell surface of mature B-lymphocytes. Background Cluster of differentiation 22 also known as CD22 is a B-cell-restricted transmembrane protein, which acts as a negative regulator of B-cell signalling. CD22 also has lectin-like adhesive properties.1 CD22 is a good target to shorten the time required for Ab production, thereby augmenting host defense against acute infectious diseases as "universal vaccination."2 Antigen Distribution The CD22 antigen is expressed on >90% of peripheral blood B-cells.1 This antigen is not present on T-cells, monocytes or macrophages. CD22 is expressed on most peripheral B-cell lymphomas and leukemias including hairy cell leukemia, but not on the immature bone marrow type of leukemia. Ligand/Receptor CD45RO, CD75 PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Immunology References & Citations1. Crocker PR et al. (2000) Immunol. 101: 342
2. Tsubata T et al. (2008) J Immunol. 180: 907
Technical Protocols Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
