Anti-Human CD22 (Inotuzumab)
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Antibody DetailsProduct DetailsReactive Species Human Host Species HEK 293 FC Effector Activity Active Immunogen Human CD22 Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% by SDS Page ⋅ ≥95% monomer by analytical SEC Formulation This biosimilar antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. State of Matter Liquid Product Preparation Recombinant biosimilar antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade biosimilar antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2-8°C Wet Ice Additional Applications Reported In Literature ? ELISA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Inotuzumab but is not covalently linked to Calich-DMH. Inotuzumab specifically recognizes CD22 on human B cells but not on murine, rat,
canine, porcine, or primate (cynomolgus and rhesus) B cells. Background N-acetyl-γ-calicheamicin is a potent, natural cytotoxic agent produced by Micromonospora echinospora that induces double-strand DNA breaks and apoptosis in rapidly proliferating cells, independent of cell cycle progression, and is therefore also of interest as a chemotherapeutic agent2. The semisynthetic derivative N-acetyl-γ-calicheamicin dimethyl hydrazide (Calich-DMH; calicheamicin) is used as an enediyne antitumor antibiotic in CD22-based chemotherapy3. Inotuzumab is composed of humanized CD22-directed monoclonal antibody G5/44 covalently attached to Calich-DMH via an acid-cleavable linker2, 4, 5, 6. The acetyl butyrate linker attaches via an amide bond to surface-exposed lysines of G5/44 and is further stabilized by two methyl groups2. When Inotuzumab binds CD22-expressing tumor cells, the inotuzumab-CD22 complex is rapidly internalized and the acidic intracellular environment triggers the release of Calich-DMH6, 7. Calich-DMH then binds to the minor groove of DNA, undergoes a structural change in its enediyne moiety that generates diradicals, and induces double-strand DNA breakage, cell cycle arrest and apoptosis2. Humanized G5/44 was derived from murine m5/44 by grafting the complementarity-determining regions plus key framework residues onto human acceptor frameworks and then expressing in Chinese hamster ovary cells4, 5. The CD22-specific targeting antibody G5/44 carries a S229P mutation in its hinge region that allows it to form stable interchain disulfide bonds and removes the potential for Fab exchange with natural IgG45. Inotuzumab has been approved for the treatment of some patients with CD22-positive B-cell precursor acute lymphoblastic leukaemia6. This research-grade biosimilar is not covalently bound to Calich-DMH. Antigen Distribution CD22 is expressed on the surface of mature B lymphocytes and their
malignant counterparts. CD22 is expressed in the cytoplasm of pro-B and pre-B cells, with
surface expression increasing in maturing B cells. CD22 expression is lost as B cells mature to
plasma cells. Ligand/Receptor CD22/CD45RO, CD75 NCBI Gene Bank ID UniProt.org Research Area Cancer . Immuno-Oncology . Immunology References & Citations1 Yilmaz M, Richard S, Jabbour E. Ther Adv Hematol. 6(5):253-261. 2015. 2 Thota S, Advani A. Eur J Haematol. 98(5):425-434. 2017. 3 Ricart AD. Clin Cancer Res. 17(20):6417-6427. 2011. 4 DiJoseph JF, Armellino DC, Boghaert ER, et al. Blood. 103(5):1807-1814. 2004. 5 DiJoseph JF, Popplewell A, Tickle S, et al. Cancer Immunol Immunother. 54:11–24. 2005. 6 Lamb YN. Drugs. 77(14):1603-1610. 2017. 7 de Vries JF, Zwaan CM, De Bie M, et al. Leukemia. 26(2):255-264. 2012. 8 DiJoseph JF, Dougher MM, Evans DY, et al. Cancer Chemother Pharmacol. 67(4):741-749. 2011. 9 Kantarjian HM, DeAngelo DJ, Stelljes M, et al. N Engl J Med. 375(8):740-753. 2016. Technical ProtocolsCertificate of Analysis |
Formats Available
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Prod No. | Description |
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C1010 | |
C1015 |
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