Anti-Human CD22 – Purified
Anti-Human CD22 – Purified
Product No.: C179
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Clone RFB-4 Target CD22 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names BL-CAM, MGC130020, SIGLEC2 Isotype IgG1 Applications FC , IHC FF |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Product Concentration 0.5 mg/ml Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This purified antibody is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828857 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this RFB-4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IHC (Frozen) Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human CD22 (Clone RFB-4) recognizes Human CD22. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Specificity: Anti-Human CD22 recognizes the (Mr 135 kDa) cytoplasmic antigen of all B-lymphocytes. The CD22 antigen is only expressed on the cell surface of mature B-lymphocytes. Background Cluster of differentiation 22 also known as CD22 is a B-cell-restricted transmembrane protein, which acts as a negative regulator of B-cell signalling. CD22 also has lectin-like adhesive properties.2 CD22 is a good target to shorten the time required for Ab production, thereby augmenting host defense against acute infectious diseases as "universal vaccination."3 Antigen Distribution The CD22 antigen is expressed on >90% of peripheral blood B-cells.2 This antigen is not present on T-cells, monocytes or macrophages. CD22 is expressed on most peripheral B-cell lymphomas and leukemias including hairy cell leukemia, but not on the immature bone marrow type of leukemia. Ligand/Receptor CD45RO, CD75 PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Immunology References & Citations1. Ebens, A. et al. (2009) Cancer Res.69(6):2358-64. Article Link 2. Crocker PR et al. (2000) Immunol. 101: 342 3. Tsubata T et al. (2008) J Immunol. 180: 907 Technical Protocols IHC FF Certificate of Analysis |
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Formats Available
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C1960 | |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
