Anti-Human CD23 - Purified - Leinco Technologies

Anti-Human CD23 – Purified

Product No.: C1601


Clone 138628 Target CD23 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Fc-epsilon-RII, Lymphocyte IgE receptor, BLAST-2 Isotype IgG1 Applications B , FC , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Mouse myeloma cell line NS0-derived recombinant human CD23/Fc epsilon RII Met150-Ser321 Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828664 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD23. A suggested method would be to stain cells expressing CD23 with 0.25 µg/106 cells in a total staining volume of 200 µl followed by PN:M1189. Western Blotting: To detect Human CD23 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CD23 is 25 and 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C1591. Additional Applications Reported In Literature ? Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.5-2.5 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of human IgE to immobilized Recombinant Human CD23/Fc epsilon RII (#123-FE) coated at 2 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Human CD23 (Clone 138628) recognizes an epitope on Human CD23. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Ligand/Receptor IgE, CD19/CD21/CD81 PubMed CD23 NCBI Gene Bank ID 2208 UniProt.org Information on Uniprot.org References & Citations View product citations for antibody C1601 on CiteAb Technical Protocols B Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD23/Fc epsilon RII Met150-Ser321

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828664

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD23. A suggested method would be to stain cells expressing CD23 with 0.25 µg/106 cells in a total staining volume of 200 µl followed by PN:M1189.
Western Blotting: To detect Human CD23 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CD23 is 25 and 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C1591.

Additional Applications Reported In Literature ?

Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.5-2.5 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of human IgE to immobilized Recombinant Human CD23/Fc epsilon RII (#123-FE) coated at 2 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human CD23 (Clone 138628) recognizes an epitope on Human CD23. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Ligand/Receptor

IgE, CD19/CD21/CD81

PubMed

CD23

NCBI Gene Bank ID

2208

UniProt.org

Information on Uniprot.org