Anti-Human CD279 (PD-1) (Nivolumab) – Dylight® 488
Anti-Human CD279 (PD-1) (Nivolumab) – Dylight® 488
Product No.: LT1211
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Product No.LT1211 Clone 5C4.B8 Target PD-1 Product Type Biosimilar Recombinant Human Monoclonal Antibody Alternate Names PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1 Isotype Human IgG1κ Applications FA , FC , IHC |
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Antibody DetailsProduct DetailsReactive Species Human/Cynomolgus Monkey Host Species Human Expression Host HEK-293 Cells FC Effector Activity Active Immunogen Human PD-1 Product Concentration 0.2 mg/ml Formulation This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only (RUO). Non-Therapeutic. Country of Origin USA Shipping Next Day 2-8°C Excitation Laser Blue Laser (493 nm) RRIDAB_2893897 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for Nivolumab biosimilar antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IHC FA Additional Reported Applications For Relevant Conjugates ? B Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Nivolumab. Clone 5C4.B8 binds to the extracellular portion of Human/Cynomolgus PD-1 and does not bind to other IgG superfamily proteins. This product is for research use only. Background Programmed cell death protein 1 (PD-1) is a protein on the surface of cells that plays a role in the maintenance of self-tolerance. PD-1 promotes self-tolerance via the down-regulation of the immune system which results in the suppression of T cell inflammatory activity. PD-L1 and PD-L2 are the two ligands known to bind PD-1. PD-L1 has increased expression in several cancers.1 PD-L2 has a more limited expression and is primarily expressed by dendritic cells and only some tumor lines. Inhibition of the interaction of PD-1 with its ligands can function as an immune checkpoint blockade through the improvement of In vitro T-cell responses and via the mediation of anti-tumor activity.2 Nivolumab disrupts the negative signal that is responsible for T-cell activation and proliferation by binding to PD-1 on activated immune cells to selectively block the interaction of the PD-1 receptor with its ligands.3 Emerging research suggests that combined blockade of PD-1 and CTLA-4, with nivolumab and ipilimumab respectively, could produce greater antitumor activity than blockade of either pathway alone.4 This cost-effective, research-grade Anti-Human CD279 (PD-1) (Nivolumab) utilizes the same variable regions from the therapeutic antibody Nivolumab making it ideal for research projects. Antigen Distribution PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. Ligand/Receptor PD-L1 and PD-L2 PubMed NCBI Gene Bank ID UniProt.org Research Area Biosimilars . Costimulatory Molecules . Immuno-Oncology . Immunology References & Citations1. Minato, N. et al. (2002) Proc Natl Acad Sci U S A. 99(19): 12293–97. 2. Korman, AJ. et al. (2014) Cancer Immunol Res. 2(9):846-56. 3. Li, Y. et al. (2016) MAbs. 8(5):951-60. 4. Wolchok, JD. et al. (2013) N Engl J Med 369(2):122-33. Technical ProtocolsFA   Certificate of Analysis |
Formats Available
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
