Anti-Human CD3 – DyLight® 488

Purified Recombinant Human CD3 (>98%)

This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This DyLight^® 488 conjugate is stable when stored at 2-8°C. Do not freeze.

Next Day 2-8°C

Blue Laser (493 nm)

IF
IHC (Frozen) The suggested concentration for this UCHT-1 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.

Clone UCHT-1 recognizes an epitope on human CD3ε.

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.

Peptide antigen bound to MHC

1. van Dongen, J. et al. (1988) Blood 71:603
2. Meuer, SC. et al. (1983) Nature 303:808
3. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329
4. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2