Anti-Human CD3 – Purified
Anti-Human CD3 – Purified
Product No.: C105
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Clone UCHT-1 Target CD3 Formats AvailableView All Product Type Monoclonal Antibody Isotype IgG1 Applications Act , FC , IF Staining , IHC FF , IP , PhenoCycler® , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Product Concentration 0.5 mg/ml Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This purified antibody is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828317 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB Additional Applications Reported In Literature ? CODEX® Activation IHC (Frozen) The suggested concentration for this UCHT-1 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone UCHT-1 recognizes an epitope on human CD3ε. Background CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition. Antigen Distribution CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells. Ligand/Receptor Peptide antigen bound to MHC PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Aliño, SF. et al. (2002) J Control Release. 83(1):133-46. PubMed 2. Yamada, T. et al. (2003) J Immunol 170:4441-9Article Link 3. Hitsuda, Y. et al. (1999) Yonago Acta medica 42:1–10 Article Link 4. van Dongen, J. et al. (1988) Blood 71:603 5. Meuer, SC. et al. (1983) Nature 303:808 6. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329 7. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2 Technical ProtocolsAct  IF Staining IHC FF  PhenoCycler®  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
