Anti-Human CD3 (Clone HIT3a) – Purified in vivo GOLD™ Functional Grade
Anti-Human CD3 (Clone HIT3a) – Purified in vivo GOLD™ Functional Grade
Product No.: C2446
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Clone HIT3a Target CD3 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names T3 Isotype Mouse IgG2a k Applications FC |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Product Concentration 1.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829543 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this HIT3a antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone HIT3a recognizes an epitope on human CD3. Background CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition. Antigen Distribution CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells. Ligand/Receptor Peptide antigen bound to MHC PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Meuer, S. C. et al. (1983) Nature 303:808
2. van Dongen, J. J. M. et al. (1988) Blood 71:603
3. Beverly, P. C. L. et al. (1981) Eur. J. Immunol. 11:329
4. Leukocyte Typing: 3rd Workshop: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2 Technical Protocols Certificate of Analysis |
Formats Available
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Prod No. | Description |
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C2446 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
