Anti-Human CD38 (Clone OKT10) – Purified in vivo GOLD™ Functional Grade
Anti-Human CD38 (Clone OKT10) – Purified in vivo GOLD™ Functional Grade
Product No.: C8000
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Clone OKT10 Target CD38 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names ADPRC1 Isotype Mouse IgG1 Applications FC |
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Antibody DetailsProduct DetailsReactive Species Rhesus Monkey ⋅ Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829852 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this OKT10 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone OKT10 recognizes an epitope on human CD38. Background CD38 is a 45kD type II transmembrane glycoprotein that plays a role in the regulation of intracellular Ca2+ by synthesizing and hydrolyzing an intracellular calcium ion mobilizing messenger. CD38 has both extracellular and intracellular functions as indicated by the release of soluble protein and the ability of membrane-bound protein to become internalized. The functional molecule is a dimer that contains the catalytic site in the central portion, and is involved in both extracellular and intracellular functions. CD38 plays a role in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a biomarker to measure the progress of a chronic lymphocytic leukemia in patients. Because CD38 is expressed on mature lymphocytes and lymphoplasmacytic cells, OKT10 can be used to study final B cell maturation. OKT10 reactivity with CD38 occurs in an inversely proportional relationship to the occurrence of Ia-like antigenic expression. Hence, these two antigens can be used as reciprocal complementary reactants for the study of mature B cell malignancies, such as CLL, multiple myeloma, and Waldenström malignancy. Antigen Distribution CD38 is present on the surface of immune cells such as CD4+, CD8+, B lymphocytes, natural killer cells, mature lymphocytes, and mature lymphoplasmacytic cells. Ligand/Receptor CD31, hyaluronic acid NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Immunology References & CitationsTechnical Protocols Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
