Anti-Human CD4 (Clone OKT-4) – Purified in vivo GOLD™ Functional Grade
Anti-Human CD4 (Clone OKT-4) – Purified in vivo GOLD™ Functional Grade
Product No.: C9200
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Clone OKT-4 Target CD4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD4; T4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 Isotype Mouse IgG2b κ Applications ELISA , FC , ICC , IF Staining , IHC FF , IHC FFPE , in vivo , IP , N , PhenoCycler® , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen Human peripheral T cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829879 Applications and Recommended Usage? Quality Tested by Leinco ELISA FC WB Additional Applications Reported In Literature ? PhenoCycler-Fusion (CODEX)® IF Staining IP IHC FF IHC FFPE ICC N Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone OKT4 recognizes Human CD4. This clone recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not interfere with each other. Background CD4 is a 55 kD single-chain type I transmembrane glycoprotein and a member of the Ig superfamily. It is involved in the recognition of MHC class II molecules. Furthermore, CD4 is the primary receptor for HIV. CD4 initiates the early phase of T-cell activation via its association with the T-cell receptor complex and protein tyrosine kinase, Lck. In addition, CD4 functions as a mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Antigen Distribution CD4 is expressed on most thymocytes, a subset of T cells, and monocytes/macrophages, and may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Crotty, S. et al. (2019) Cell Rep 29(7):1756-66. 2. Ploss, A. et al. (2018) Nat Commun 9(1):5031 3. Lone, YC. et al. (2017) PLoS One.12(4): e0173754. Technical Protocols  ICC IF Staining IHC FF IHC FFPE   N PhenoCycler®  Certificate of Analysis |
Formats Available
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
