Anti-Human CD8 – Purified
Anti-Human CD8 – Purified
Product No.: C117
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Clone UCHT-4 Target CD8 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names T8, Leu2 Isotype IgG2a Applications FC , IHC FF |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Thymocytes and Sézary T cells Product Concentration 0.5 mg/ml Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This purified antibody is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2828352 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this UCHT-4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IHC (Frozen) Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone UCHT-4 recognizes human CD8. Background CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes. Antigen Distribution CD8 is expressed on blood lymphocytes, a subset of NK cells, and thymocytes. Persons with HIV exhibit increased levels of CD8+ lymphocytes. Ligand/Receptor MHC Class I molecules PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Parnes, J. R. et al. (1989) Adv. Immunol. 44:265
2. Reinherz, E. L. et al. (1980) J. Immunol. 124:1301
3. Fischer, A. et al. (1983) Immunology 48:177
4. Merkenschlanger, M. et al. (1988) Eur. J. Immunol. 18:1653
5. Leukocyte Typing: 3rd Workshop: Code No. 567; 4th Workshop: Code No. N31 Technical Protocols IHC FF Certificate of Analysis |
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Formats Available
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Prod No. | Description |
|---|---|
C2068 | |
C117 | |
C120 | |
C118 | |
C119 | |
C2060 | |
C2061 | |
C2062 | |
C2064 | |
C2066 | |
C366 | |
C312 | |
C666 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
