Anti-Human CXCL1 (Clone 20326)
Anti-Human CXCL1 (Clone 20326)
Product No.: C1393
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Clone 20326 Target CXCL1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names GRO1, GROa, MGSA, MGSA Alpha, NAP-3, SCYB1 Isotype IgG2b Applications CyTOF® , ELISA Cap , FC , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human CXCL1 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828450 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:G145 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:G125. Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCL1. A suggested method would be to stain cells expressing CXCL1 with 0.25 µg per 1 - 5 x 106 cells in a total staining volume of ≤ 200 µl followed by PN:M1189. Western Blotting: To detect Human CXCL1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL1 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G125. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human CXCL1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CXCL1 (at 10 ng/ml) is 3-15 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human CXCL1 (Clone 20326) recognizes an epitope on Human CXCL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody exhibits approximately 20% cross-reactivity with rhCXCL2/GROβ and rhCXCL3/GROγ, and no cross-reactivity with other chemokines tested. Background Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).1 CXCL1 is expressed by macrophages, neutrophils and epithelial cells.2 CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.3,4 PubMed Research Area Chemokine References & Citations1. Sager, R. et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732 2. Grotendorst, GR. et al. (1990) Mol. Cell. Biol. 10: 5596 3. Miller, RH. et al. (2002) Cell 110: 373 4. Richmond, A. et al. (2000) J. Invest. Dermatol. 115: 234 Technical ProtocolsCyTOF®   N  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
