Anti-Human CXCL7 (Clone 59418)
Anti-Human CXCL7 (Clone 59418)
Product No.: N139
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Clone 59418 Target CXCL7 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Chemokine (C-X-C Motif) Ligand 7, PPBP, PBP, B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, LA-PF4, LDGF, MDGF, Neutrophil Activating Peptide 2 (NAP-2), SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1 Isotype IgG1 Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human CXCL7 (Accession # P02775) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831403 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:N124 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:N119. Western Blotting: To detect Human CXCL7 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL7 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:N119. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human NAP-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human NAP-2 (at 1 ng/ml) is 15 - 20 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human Chemokine (C-X-C Motif) Ligand 7 (CXCL7) (Clone 59418) recognizes an epitope on Human CXCL7. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background CXC chemokine ligand 7 is a small cytokine belonging to the CXC chemokine family. It is a protein that is released in large amounts from platelets following their activation.1 It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.2,3 PubMed Research Area Chemokine References & Citations1. Poncz, M. et al. (1991) J Biol Chem. 266: 5785 2. Walz, D. et al. (1983) Proc Natl Acad Sci USA 80: 765 3. Carter-Su, C. et al. (1985) Biochemistry 24: 1762 Technical Protocols N  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
