Anti-Human DAD1

PN:D316

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human DAD1 recognizes an epitope near the C-terminus of human and mouse DAD1. This polyclonal antibody was purified using affinity chromatography.

Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death¹, a process important for normal organism development and tissue homeostasis.² DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity.^3,4 Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death.^5,6 Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas⁷, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.

1. Nakashima, T. et al. (1993) Mol. Cell Biol.13:6367-74.
2. Stellar, H. (1995) Science 267:1445-9.
3. Gilmore, R. and Kelleher, DJ. (1997) Proc. Natl. Acad. Sci. USA 94:4994-9.
4. Sanjay, A. et al. (1998) J. Biol. Chem. 273:26094-9.