Anti-Human DDX3 (NT)

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human DDX3 recognizes an epitope near the N-terminus of Human and Mouse DDX3. This polyclonal antibody was purified using affinity chromatography.

DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases.^1,2 DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome.³ DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation.⁴ It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.

1. Linder, P. et al. (1989) Nature 337:121 2. Park, S. H. et al. (1998) Cytogenet. Cell Genet. 81:178 3. Lahn, B. T. et al. (1997) Science 278:675 4. Abdelhaleem, M. (2005) Clin. Biochem. 38:499